MACROPOROUS HYDROPHOBIC CLOTH (POLYMACRON) AS A SOLID-PHASE FOR NUCLEIC-ACID PROBE HYBRIDIZATIONS

Authors
BLAIS BW PHILLIPPE LM
Citation
Bw. Blais et Lm. Phillippe, MACROPOROUS HYDROPHOBIC CLOTH (POLYMACRON) AS A SOLID-PHASE FOR NUCLEIC-ACID PROBE HYBRIDIZATIONS, Biotechnology techniques, 9(5), 1995, pp. 377-382
Citations number
8
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
5
Year of publication
1995
Pages
377 - 382
Database
ISI
SICI code
0951-208X(1995)9:5<377:MHC(AA>2.0.ZU;2-I
Abstract
Polyester cloth (polymacron) was employed as a solid phase onto which polymerase chain reaction (PCR) products from the Listeria monocytogen es hlyA gene were dot blotted and sensitively detected by hybridizatio n with nucleic acid probes. This inexpensive solid phase exhibited a h igh DNA binding capacity and provided ease of handling and washing bet ween each reaction step, resulting in simple hybridization procedures for PCR product detection.