EFFECT OF FRUCTOSE AND GLUCOSE SUPPLEMENTATION ON INVERTASE MEDIATED SYNTHESIS OF OLIGOSACCHARIDES FROM SUCROSE

The total amount of novel oligosaccharides synthesized by beta-D-fruct ofuranosidase at pH 7.5 increased three-fold using a medium composed o f 1.2M sucrose, 0.5M fructose and 0.1M glucose, as compared to that wi th only 1.8M sucrose solution. Using 0.6M of the three sugars did not increase yield but reduced rate of sucrose hydrolysis by 72.7%. Synthe sis of fructosyl/glucosyl oligosaccharides based on beta-fructofuranos idase mediated transglycosylation is enhanced by supplementation of su crose solution with appropriate concentrations fructose and glucose.