INCREASE IN STABILITY OF XYLANASE FROM AN ALKALOPHILIC THERMOPHILIC BACILLUS (NCIM-59)

Authors
NATH D RAO M
Citation
D. Nath et M. Rao, INCREASE IN STABILITY OF XYLANASE FROM AN ALKALOPHILIC THERMOPHILIC BACILLUS (NCIM-59), Biotechnology letters, 17(5), 1995, pp. 557-560
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
17
Issue
5
Year of publication
1995
Pages
557 - 560
Database
ISI
SICI code
0141-5492(1995)17:5<557:IISOXF>2.0.ZU;2-X
Abstract
The cellulase-free xylanase from an alkalophilic thermophilic Bacillus was stable at pH 7.0 to 10.0 at 50 degrees for 3 days. At 60 degrees the enzyme showed a decrease in stability with a half- life of 3 h. Ad dition of various additives had no effect on the enzyme stability at 6 0 degrees. Glycine (0.5M) increased-the enzyme half-life 6-fold at pH 7.0 to 9.0 and at 60 and 70 degrees. Xylan could offer protection agai nst thermoinactivation of the xylanase at pH 7.0 and 8.0 at 60 degrees and only a marginal increase at pH 9.0 at 70 degrees was observed.