PRECIPITATION REACTIONS WITH NEWCASTLE-DISEASE VIRUS

Acid precipitated and detergent treated Newcastle disease virus (NDV) antigen was prepared and characterised using the agar gel precipitatio n (AGP) test. The detergent treated NDV antigen was used to screen ant ibodies to NDV and the results compared with the conventional haemaggl utination inhibition (HI) test titres. AGP test could detect NDV antib odies in serum samples when the corresponding HI titres were 1:8 and a bove. Detergent treatment of Newcastle disease virus greatly reduced i ts haemagglutinating ability. Simultaneous detection of antibodies to NDV and infectious bursal disease virus in AGP test was attempted and found successful.