SIMULTANEOUS DEMONSTRATION OF MICROSCOPIC ANATOMY OF BRAIN-STEM, NISSL SUBSTANCE, LIPOFUSCIN AND CYTOLOGICAL CHANGES USING A COMBINATION METHOD

The proposed combination of three methods facilitates the of microscop ic anatomy, Nissl content of neurons, lipofuscin accumulation and asso ciated cytopathological changes, if any, in one brain section. Formald ehyde fixed paraffin sections were stained with a modified Weil's meth od, cresyl violet and pyronin Y in sequence, dehydrated, cleared and m ounted in DPX. The slides were studied under a fluorescence microscope . The fibre system stained grey. The Nissl substance and lipofuscin st ained violet and pink, respectively. On exposure to UV light, the pink stained pigment emitted golden-yellow fluorescence, resembling the au to-fluorescence of unstained lipofuscin. The final results were remark ably reproducible.