BENZOTHIAZOLE DERIVATIVES AS SUBSTRATES FOR ALKALINE-PHOSPHATASE ASSAY WITH FLUORESCENCE AND CHEMILUMINESCENCE DETECTION

Authors
SASAMOTO K DENG G USHIJIMA T OHKURA Y UENO K
Citation
K. Sasamoto et al., BENZOTHIAZOLE DERIVATIVES AS SUBSTRATES FOR ALKALINE-PHOSPHATASE ASSAY WITH FLUORESCENCE AND CHEMILUMINESCENCE DETECTION, Analyst, 120(6), 1995, pp. 1709-1714
Citations number
16
Categorie Soggetti
Chemistry Analytical
Journal title
Analyst → ACNP
ISSN journal
00032654
Volume
120
Issue
6
Year of publication
1995
Pages
1709 - 1714
Database
ISI
SICI code
0003-2654(1995)120:6<1709:BDASFA>2.0.ZU;2-Y
Abstract
Unique fluorogenic and chemiluminescence substrates for alkaline phosp hatase (ALP), 2-(N-phenylphthalimidyl)benzothiazolyl-5-phosphate and - 1',4'-dionephthalazyl)benzothiazolyl-5-phosphate, respectively, that c ontain a highly fluorescent benzothiazole moiety as the fluorophore we re synthesized and their applicabilities to the assay of ALP were exam ined. The chemiluminogenic substrate exhibited a linear chemiluminesce nce response to the amount of ALP in the range 0-20 pmol.