Acoustic backscatter has produced spectacular images of internal ocean
processes for nearly two decades, but interpretation of the images re
mains ambiguous because several mechanisms can generate measurable bac
kscatter. The authors present what is thought to be the first simultan
eous measurements of calibrated acoustic returns and turbulent microst
ructure, collected in a set of 20-m-tall billows. The observations are
from Admiralty Inlet, a salt-stratified tidal channel near Puget Soun
d. Scattering due to turbulent microstructure alone is strong enough t
o explain the measured backscatter at specific sites within the billow
s. Existing formulations underestimate the strength of acoustic backsc
atter from turbulent microstructure. Due to a misinterpretation of the
high-wavenumber temperature spectrum, some previous formulations unde
restimate the differential scattering cross section (delta) when scatt
ering from the viscous-convective subrange. Also, the influence of sal
inity on refractive-index fluctuations tan be as large as or greater t
han that of temperature when the density stratification is dominated b
y salinity. Using temperature alone to estimate delta in coastal and e
stuarine waters may lead to significant underestimates. A simple formu
lation is derived that takes these two factors into account. Because o
f high ambient scattering from zooplankton in Admiralty Inlet, the aco
ustic data are conditionally sampled along modeled profiler trajectori
es to avoid using bulk statistics. Scalar dissipation is greatest in t
he bounding surfaces of the billows, consistent with these surfaces pr
oducing the most intense scattering. Acoustic backscatter can be used
to remotely sense the spatial structure of scalar dissipation in turbu
lent events where delta due to turbulent microstructure exceeds the ba
ckground level set by scattering from biology. In lakes and the deep o
cean where scattering from zooplankton is expected to be negligible, s
cattering from microstructure may be the dominant mechanism. The large
st uncertainties in the comparison result from the very large differen
ce in sampling volume of the acoustic system and microstructure profil
er.