S. Fujita et al., ISOLATION AND CHARACTERIZATION OF 2 PHLOROGLUCINOL OXIDASES FROM CABBAGE (BRASSICA-OLERACEA L), Journal of agricultural and food chemistry, 45(1), 1997, pp. 59-63
Two isozymes (F-IB and F-II) of phloroglucinol oxidase (PhO) in cabbag
e (Brassica oleracea L.) were purified from cabbage. The purified enzy
mes were found to be in a homogeneous state by polyacrylamide gel elec
trophoresis (PAGE) and sodium dodecyl sulfate (SDS)-PAGE. The molecula
r weights of F-IB and F-II were estimated to be similar to 43 000 and
32 000, respectively, by SDS-PAGE. Both purified enzymes only oxidized
1,3,5-trihydroxybenzenes, such as phloroglucinol and phloroglucinolca
rboxylic acid. Both enzymes also had strong peroxidase (POD) activity.
The pH optima of PhO and POD of F-IB were 8.0 and 6.7, respectively,
and those of F-II were 7.4 and 6.7, respectively. Activities of both F
-IB and F-II were stable in the pH range 6-11 at 5 degrees C for 20 h,
and were markedly inhibited by sodium diethyldithiocarbamate and pota
ssium cyanide. MnCl2 markedly activated the PhO activity of F-LB and F
-II, but strongly inhibited their POD activity.