We cloned the full genomic DNA of yeast (Saccharomyces cerevisiae) O-6
-methylguanine-DNA methyltransferase (MGMT) gene and examined its expr
ession. The expression of yeast MGMT gene is decreased when cells reac
h stationary phase and cannot be induced by the pretreatment with alky
lating agents, methylmethanesulfonate (MMS) or N-methyl-N'-nitroso-N-n
itrosoguanidine (MNNG). The transcription initiation site was determin
ed by primer extension analysis. This analysis showed that the authent
ic start codon is the ATG at position +32 from transcription initiatio
n site.