RESTRICTED TISSUE-SPECIFIC BUT CORRECT DEVELOPMENTAL EXPRESSION MEDIATED BY A SHORT HUMAN ALPHA-1AT PROMOTER FRAGMENT IN TRANSGENIC MICE

Authors
YULL FE WALLACE RM CLARK AJ
Citation
Fe. Yull et al., RESTRICTED TISSUE-SPECIFIC BUT CORRECT DEVELOPMENTAL EXPRESSION MEDIATED BY A SHORT HUMAN ALPHA-1AT PROMOTER FRAGMENT IN TRANSGENIC MICE, Transgenic research, 4(1), 1995, pp. 70-74
Citations number
30
Categorie Soggetti
Genetics & Heredity
Journal title
Transgenic research → ACNP
ISSN journal
09628819
Volume
4
Issue
1
Year of publication
1995
Pages
70 - 74
Database
ISI
SICI code
0962-8819(1995)4:1<70:RTBCDE>2.0.ZU;2-7
Abstract
The tissue-specific and developmental pattern of expression controlled by the proximal promoter (position -348 to +15) derived from the huma n alpha-1-antitrypsin (h alpha 1AT) gene was studied in transgenic mic e. The short promoter segment was linked to the chloramphenicol acetyl transferase (CAT) reporter gene. The transgene showed highly specific expression in the liver and the correct developmental pattern of regul ation. Interestingly, this short promoter targets expression to the li ver with a greater specificity than that reported for larger alpha 1AT promoter fragments.