HIGH-ENERGY PHOSPHATE METABOLITES OBSERVED BY NMR IN INFECTIVE LARVAEOF HAEMONCHUS-CONTORTUS

Phosphorus resonances consistent with phosphoarginine and ATP were obs erved in the in vivo P-31-nuclear magnetic resonance (NMR) spectra of infective larvae of Haemonchus contortus. The level of phosphoarginine quickly declined when nematode suspensions were purged with nitrogen and was restored upon return to aerobic conditions. Saturation transfe r NMR demonstrated forward and reverse exchange of phosphorus between phosphoarginine and ATP.