The algal fermentation conditions for the hydrogen production were inv
estigated to improve the photobiological hydrogen production by a comb
ination of a marine green alga Chlamydomonas sp. MGA1G1 and a marine p
hotosynthetic bacterium Rhodopseudomonas sp. W-1S. The scaling-up of o
ur biophotolysis system with algal-bacterial combination was carried o
ut. The stably sustained starch accumulation and degradation in the al
gal cells and hydrogen photoproduction from algal fermentation product
s by photosynthetic bacteria occurred in an alternating light-dark cyc
le.