HUMAN LYMPHOCYTOTOXIC MONOCLONAL AUTOANTIBODIES FROM A HIGHLY SENSITIZED RENAL DIALYSIS PATIENT

A panel of 5 human monoclonal autolymphocytotoxic antibodies (IRM-3, I RM-4, IRM-7, IRM-8, and IRM-10) of the IgM class was established from a highly sensitized renal dialysis patient (IRM), by the generation of mouse-human heterohybridomas. This panel was screened for reactivity against foreign and autoantigens by ELISA, and for reactivity against different tissue sections and HEp-2 slide preparations by indirect imm unofluorescence. Cytotoxicity screening of heterohybridoma supernatant s gave broad panel reactivity profiles, being cytotoxic against B cell s from patient IRM and also against most B cells tested and less react ive with chronic lymphocytic leukemia B cells; T cells were the least sensitive target. Immunoblotting showed that monoclonal IRM displayed some heterogeneity in their binding profiles, although all of them rec ognized a cellular structure of 26 kDa. None of the heterohybridoma ce ll lines exhibited cytoplasmic nor surface staining with an anti-CD5 m Ab. Results obtained showed that all the autolymphocytotoxic mAbs gene rated were also able to react against certain nuclear and cytoplasmic self-structures as well as foreign compounds. Monoclonal antibody IRM- 7 and, to a lesser degree, IRM-10 exhibited multispecific properties s imilar to those observed for polyreactive or natural antibodies.