HERPES BUFFER - ASSESSMENT OF OPTIMUM CONCENTRATION TO MAINTAIN PH INVERO CELL-CULTURE

Vero Cell line, the African green monkey (Cercopithecus aethipps sabeu s) kidney cell line is widely used for the propagation of wide range o f animal viruses (Mac Farlane and Somerville, 1969). The maintenance o f pH of the medium for cell line culture, especially in virus titratio n experiments requiring longer days of incubation, is found to be diff icult. Th ou gh bicarbonate - CO2 buffer system in tissue culture (Tc) medium is routinely adopted, addition of supplementary organic buffer s like - glycerophosphate or HEPES (N-2-hydroxy ethyl piperazine-n - 2 -ethane sulfonic acid) is advocated for effective maintenance of pH (H am and Mc Keehan, 1979). In the present study, various mM concentratio n of HEPES in tissue culture medium was used for Vero Cell culture to arrive at optimum concentration required for effective maintenance of pH when cultures are incubated in an ordinary laboratory incubator.