A. Ohno et al., PRODUCTION OF A LIPOPEPTIDE ANTIBIOTIC, SURFACTIN, BY RECOMBINANT BACILLUS-SUBTILIS IN SOLID-STATE FERMENTATION, Biotechnology and bioengineering, 47(2), 1995, pp. 209-214
Production of a lipopeptide antibiotic, surfactin, in solid state ferm
entation (SSF) on soybean curd residue, Okara, as a solid substrate wa
s carried out using Bacillus subtilis MI113 with a recombinant plasmid
pC112, which contains Ipa-14, a gene related to surfactin production
cloned at our laboratory from a wild-type surfactin producer, B. subti
lis RB14. The optimal moisture content and temperature for the product
ion of surfactin were 82% and 37 degrees C, respectively. The amount o
f surfactin produced by MI113 (pC112) was as high as 2.0 g/kg wet weig
ht, which was eight times as high as that of the original B. subtilis
RB14 at the optimal temperature for surfactin production, 37 degrees C
. Although the stability of the plasmid showed a similar pattern in bo
th SSF and submerged fermentation (SMF), production of surfactin in SS
F was 4-5 times more efficient than in SMF. (C) 1995 John Wiley and So
ns, Inc.