THE NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR ALPHA-2 SUBUNIT GENE PROMOTER IS ACTIVATED BY THE BRN-3B POU FAMILY TRANSCRIPTION FACTOR AND NOT BY BRN-3A OR BRN-3C
Ngn. Milton et al., THE NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR ALPHA-2 SUBUNIT GENE PROMOTER IS ACTIVATED BY THE BRN-3B POU FAMILY TRANSCRIPTION FACTOR AND NOT BY BRN-3A OR BRN-3C, The Journal of biological chemistry, 270(25), 1995, pp. 15143-15147
The regulatory region of the neuronal nicotinic acetylcholine receptor
alpha 2 subunit gene, which contains six copies of the octamer-relate
d sequence CCCCATGCAAT, is activated by the Brn-3b POU family transcri
ption factor but not by the closely related factors Brn-3a and Brn-3c.
This effect is in contrast to the previously documented inhibitory ef
fect of Brn-3b on octamer-containing promoters that are activated by B
rn-3a and Brn-3c. Activation of the alpha 2 gene by Brn-3b requires th
at both the POU domain and other N-terminal sequences are derived from
Brn-3b and is dependent on the intactness of the alpha 2 gene regulat
ory region, being lost in truncated derivatives containing one, two, o
r four copies of the octamer-related sequence. Surprisingly, however,
these truncated derivatives are activated by Brn-3c. These effects are
discussed in terms of both the influence of the target sequence and i
ts context in the promoter on activation by the various forms of Brn-3
as well as of the processes that restrict expression of the alpha 2 s
ubunit gene to a few cells in the nervous system.