REPLACEMENT OF NATIVE ANIMAL SERA BY GROW TH-STIMULATING PROTEINS IN CULTURING OF MAMMALIAN-CELLS

Authors
PODCHERNYAYEVA RY MELNICHENKO YI KONDU EI
Citation
Ry. Podchernyayeva et al., REPLACEMENT OF NATIVE ANIMAL SERA BY GROW TH-STIMULATING PROTEINS IN CULTURING OF MAMMALIAN-CELLS, Voprosy virusologii, 40(1), 1995, pp. 39-41
Citations number
NO
Categorie Soggetti
Virology
Journal title
Voprosy virusologii → ACNP
ISSN journal
05074088
Volume
40
Issue
1
Year of publication
1995
Pages
39 - 41
Database
ISI
SICI code
0507-4088(1995)40:1<39:RONASB>2.0.ZU;2-T
Abstract
The authors recommend to replace nonstandard native animal sera by gro wth-stimulating proteins isolated from the sera of cattle, northern de ers, pigs, and sheep by fractionation with polyethylene glycol aqueous solutions (mol. m 6000 dalton). Growth-stimulating proteins are condu cive to active proliferation of primary and continuous cell cultures a t various methods of culturing. Growth-stimulating proteins were for t he first time added to nutrient media during culturing of mammalian ce lls on microcarriers.