Ry. Podchernyayeva et al., REPLACEMENT OF NATIVE ANIMAL SERA BY GROW TH-STIMULATING PROTEINS IN CULTURING OF MAMMALIAN-CELLS, Voprosy virusologii, 40(1), 1995, pp. 39-41
The authors recommend to replace nonstandard native animal sera by gro
wth-stimulating proteins isolated from the sera of cattle, northern de
ers, pigs, and sheep by fractionation with polyethylene glycol aqueous
solutions (mol. m 6000 dalton). Growth-stimulating proteins are condu
cive to active proliferation of primary and continuous cell cultures a
t various methods of culturing. Growth-stimulating proteins were for t
he first time added to nutrient media during culturing of mammalian ce
lls on microcarriers.