A dot enzyme-linked immunosorbent assay (dot-ELISA) was developed usin
g excretory-secretory (ES) antigens of Fasciola gigantica adult worms
and freeze-thaw, antigen (AFT) of adult Schistosoma mansoni worms. Spe
cific IgG and IgM antibodies were simultaneously investigated in sera
from 17 febrile eosinophilic hospitalized patients and 20 healthy cont
rols. Dot-ELISA was shown useful and could differentiate fascioliasis
from schistosomiasis prior to parasite egg detection in patient's stoo
l. This assay is field and laboratory applicable.