Ku. Klages et Hw. Morgan, CHARACTERIZATION OF AN EXTREMELY THERMOPHILIC SULFUR-METABOLIZING ARCHAEBACTERIUM BELONGING TO THE THERMOCOCCALES, Archives of microbiology, 162(4), 1994, pp. 261-266
An extremely thermophilic, obligately anaerobic, sulphur-metabolizing
archaebacterium of the order Thermococcales, previously isolated from
a thermal pool at Kuirau Park, Rotorua, New Zealand, partially describ
ed, and designated isolate ANI,Thermococcales was further characterize
d. The isolate was a regular coccus of 0.5-2.0 mm in diameter, was str
ictly anaerobic, chemoorganotrophic, and fermentative. Peptone, yeast
extract, or casein served as carbon and nitrogen source, and a variety
of amino acids and glucose, but not organic acids, carbohydrates, or
other sugars supported growth in the presence of peptone (0.1%). Major
metabolic end products were H-2, sulphide, acetate, isobutyrate, and
isovalerate/2-methylbutyrate. Isolate ANI had a temperature optimum of
75-80 degrees C, a pH optimum of 7.4, and a sodium chloride concentra
tion optimum of 50 mM. No growth was observed in the absence of sodium
chloride (or lithium chloride) and sulphur (or cystine or oxidized gl
utathione).