PGE(2) INDUCES C-FOS EXPRESSION BY A CAMP-INDEPENDENT MECHANISM IN GLOMERULAR MESANGIAL CELLS

Prostanoids induce expression of several immediate-early genes, but th e molecular mechanisms underlying these responses remain poorly charac terized. We studied induction of the proto-oncogene c-fos by PGE(2) in mesangial cells as a model of gene regulation by prostanoids. PGE(2) induced marked and transient accumulation of c-fos mRNA. Addition of e xogenous 8-bromo-cAMP or forskolin failed to induce c-fos mRNA, sugges ting that activation of an EP2 receptor linked to adenylate cyclase di d not account for induction of c-fos by PGE(2). These data contrast wi th previous experiments in NIH 3T3 cells in which PGE(2) induced c-fos by a cAMP-dependent mechanism. Depletion of protein kinase C blocked induction of c-fos mRNA by PGE(2), whereas a protein tyrosine kinase i nhibitor had no effect. We further showed that PGE(2) induces the c-fo s gene by increasing the transactivating capacity of the serum-respons e element. Transient transfections with a CAT fusion gene driven by an AP-1 cis-element demonstrated that although PGE(2) markedly induced c -fos, PGE(2) did not increase AP-1-driven transcriptional responses. E lectrophoretic gel mobility shift assays revealed that PGE(2) failed t o increase binding of AP-1 complexes to a consensus AP-1 DNA sequence. Taken together, these experiments provide evidence for a cAMP-indepen dent, protein kinase C-dependent pathway linking a PGE(2) receptor on the plasma membrane to transcriptional activation in the nucleus. Regu lation of gene transcription by PGE(2) probably involves c-fos inducti on without concomitant activation of AP-1. (C) 1994 Academic Press, In c.