We used restriction enzyme analysis of polymerase chain reaction ampli
fied mitochondrial DNA to assess genetic differentiation of two subspe
cies of walruses: Atlantic walruses (Odobenus rosmarus rosmarus) from
Greenland and Pacific walruses (O. r. divergens) from the Chukchi Sea.
Each subspecies has distinct monophyletic mitochondrial DNA haplotype
s. Mitochondrial DNA sequence divergence between Atlantic and Pacific
subspecies was 1.0-1.6%. Several haplotypes were observed for each sub
species. Haplotype frequencies varied among sampling locations of Atla
ntic walruses, suggesting that mitochondrial DNA may be useful for wal
rus stock identification.