REGENERATION OF TRANSGENIC, MICROSPORE-DERIVED, FERTILE BARLEY

Authors
JAHNE A BECKER D BRETTSCHNEIDER R LORZ H
Citation
A. Jahne et al., REGENERATION OF TRANSGENIC, MICROSPORE-DERIVED, FERTILE BARLEY, Theoretical and Applied Genetics, 89(4), 1994, pp. 525-533
Citations number
41
Categorie Soggetti
Genetics & Heredity
Journal title
Theoretical and Applied Genetics → ACNP
ISSN journal
00405752
Volume
89
Issue
4
Year of publication
1994
Pages
525 - 533
Database
ISI
SICI code
0040-5752(1994)89:4<525:ROTMFB>2.0.ZU;2-B
Abstract
We have developed a system for the biolistic transformation of barley using freshly-isolated microspores as the target tissue. Independent t ransformation events led, on average, to the recovery of one plant per 1x10(7) bombarded microspores. Putative transformants have been regen erated using phosphinothricin as a selective agent. R(0) plants have b een transferred to soil approximately 2 months after bombardment. Inte gration of the marker genes bar and uidA has been confirmed by Souther n analysis. The marker genes are inherited in all progeny plants confi rming the expected homozygous nature of the R(0) plants.