B. Richterruoff et al., DEGRADATION OF THE YEAST MAT-ALPHA-2 TRANSCRIPTIONAL REGULATOR IS MEDIATED BY THE PROTEASOME, FEBS letters, 354(1), 1994, pp. 50-52
Rapid degradation of specific regulatory proteins plays a role in a wi
de range of cellular phenomena, including cell cycle progression and t
he regulation of cell growth and differentiation. A major mechanism of
selective protein turnover in vivo involves a large multi-subunit pro
tease known as the proteasome or multi-catalytic proteinase. At the sa
me time, the degradation of many cellular proteins requires their cova
lent ligation to the polypeptide ubiquitin. Here we show that the yeas
t S. cerevisiae MAT alpha 2 repressor, which is known to be ubiquitiny
lated in vivo, requires the proteasome for its rapid intracellular pro
teolysis.