NUCLEOTIDE RECEPTORS IN HEPATIC STELLATE CELLS OF THE RAT

When hepatic stellate cells were stimulated by UTP, ATP, or ADP, cellu lar levels of inositol phosphates significantly increased (UTP > ATP > ADP > 5'-0-(3-thiotriphosphate). Thirty min after incubation with 100 mu M of UTP, ATP, or ADP, levels of inositol monophosphate increased to 1318 +/- 116, 616 +/- 87 and 591 +/- 234% of control levels, respec tively, with concomitant increase in the production of inositol trisph osphate and bisphosphate. These nucleotides transiently increased the [Ca2+] of fura-2-loaded stellate cells. Moreover, UTP, ATP, ADP and ad enosine 5'-0-(3-thiotriphosphate) were able to induce contraction of s tellate cells as detected using the silicone-rubber membrane method. T hese results suggested that hepatic stellate cells have nucleotide rec eptors which react predominantly with extracellular UTP and ATP and tr igger the receptor-mediated contraction of the cells.