H. Nilsson et al., LASER-INDUCED FLUORESCENCE IN MALIGNANT AND NORMAL TISSUE IN MICE INJECTED WITH 2 DIFFERENT CAROTENOPORPHYRINS, British Journal of Cancer, 70(5), 1994, pp. 873-879
Laser-induced fluorescence (LIF) was used to characterise the localisa
tion of an intravenously administered trimethylated carotenoporphyrin
[CP(Me)(3)] and a trimethoxylated carotenoporphyrin [CP(OMe)(3)] in an
intramuscularly transplanted malignant tumour (MS-2 fibrosarcoma) and
healthy muscle in female Balb/c mice, 3, 24, 48 and 96 h post injecti
on. The fluorescence was induced with a dye laser pumped by a nitrogen
laser, emitting light at 425 nm. The fluorescence spectra were record
ed in the region 455-760 nm using a polychromator equipped with an ima
ge-intensified CCD camera. The tumour/peritumoral muscle ratio was abo
ut 5:1 for CP(Me), and about 6:1 for CP(OMe)(3) in terms of the backgr
ound-free fluorescence intensity, which peaked at about 655 nm. By inc
luding the endogenous tissue fluorescence, the contrast was further en
hanced by a factor of approximately 2.