C. Genot et al., MEASUREMENT OF PHOSPHOLIPID OXIDATION IN MODEL MEMBRANES BY DETERMINATION OF OXYGEN-CONSUMPTION WITH A SEMIAUTOMATIC POLAROGRAPHIC METHOD, Sciences des aliments, 14(5), 1994, pp. 673-682
Among methods for studying lipid oxidation, the measurement of oxygen
consumption is rarely used, probably because it is difficult to proces
s the data obtained as traces on paper recorder. With the described sy
stem, the data obtained from two oxygen meters are collected and analy
sed to study catalysed phospholipid oxidation in liposomes. Oxygen upt
ake and rate of oxygen consumption at different times, maximum rate an
d apparent pseudo first order constants in fast and slow stages of the
reaction are easily obtained with good reproducibility on homogeneous
series of fresh phospholipids. When the phospholipids are already oxi
dised, the shape of the curve is modified as well as the calculated pa
rameters. As compared to PIPES buffer, phospholipid oxidation is inhib
ited by cacodylate buffer in the first stages of the reaction, and enh
anced later on. The antioxidant effects of alpha-tocopherol and BHT ar
e clearly shown.