PHYLOGENY AND BIOTRANSFORMATION .5. BIOTRANSFORMATION OF ISOPINOCAMPHEOL

Biotransformation of isopinocampheol with 100 bacterial and fungal str ains yielded 1-, 2-, 4-, 5-, 7-, 8- and 9-hydroxy-isopinocampheol besi des three rearranged monoterpenes, one of them bearing the novel isoca rane skeleton. A pronounced enantioselectivity between (+)- and (-)-is opinocampheol was observed. The phylogenetic position of the individua l strains could be seen in their ability to form the products from (+) -isopinocampheol. The formation of 1,3-dihydroxypinane is a domain of bacteria, while 3,5- or 3,7-dihydroxypinane was mainly formed by fungi , especially those of the phylum Zygomycotina. The activity of Basidio mycotina towards oxidation of isopinocampheol was rather low. Such inf ormations can be used in a more effective selection of strains for scr eening.