Jfjm. Vandenheuvel et al., CHARACTERISTICS OF A RESISTANCE-BREAKING ISOLATE OF POTATO VIRUS-Y CAUSING POTATO-TUBER NECROTIC RINGSPOT DISEASE, European journal of plant pathology, 100(5), 1994, pp. 347-356
An Austrian isolate of potato virus Y(NTN), the causal agent of potato
tuber necrotic ringspot disease (PTNRD), was serologically compared w
ith seven Dutch PVY(N) isolates. Using polyclonal and monoclonal antib
odies, it was found indistinguishable from PVY(N). Determination of th
e nucleotide sequence of the coat protein cistron and comparison of th
e deduced amino acid sequence with coat protein sequences of other pot
yviruses revealed a high level of homology with PVY(N) coat protein se
quences. This confirmed the close taxonomic relationship of PVY(NTN) w
ith the PVY(N) subgroup of potato virus Y. PVY(NTN) is able to overcom
e all resistance genes known so far in commercial potato cultivars. Re
markably, transgenic PVY-protected tobacco plants are also resistant t
o PVY(NTN) infection upon mechanical and aphid-mediated inoculation. T
hese experiments indicate that genetically engineered resistance offer
s great potential in protection of potato to new aggressive strains of
PVY(N).