Rs. Sohal et al., EFFECT OF AGE AND CALORIC RESTRICTION ON DNA OXIDATIVE DAMAGE IN DIFFERENT TISSUES OF C57BL 6 MICE/, Mechanism of ageing and development, 76(2-3), 1994, pp. 215-224
The objective of this study was to explore the role of molecular oxida
tive damage and caloric intake in the aging process. The concentration
of 8-hydroxydeoxyguanosine (8-OHdG), a product of DNA oxidation, was
compared in five different tissues of mice (skeletal muscle, brain, he
art, liver and kidney) as a function of age and in response to dietary
restriction. A comparison of 8- and 27-month-old mice indicated that
the age-related increase in 8-OHdG concentration was greater in skelet
al muscle, brain and heart, which are primarily composed of long-lived
, post-mitotic cells, than in liver and kidney, which consist of slow-
dividing cells. Dietary restricted (DR) mice kept on 60% caloric intak
e as compared to the ad libitum-fed (AL) mice showed a lower concentra
tion in 8-OHdG content in all the tissues compared to AL mice. The DR-
related amelioration of DNA oxidative damage was greater in the post-m
itotic tissues compared to those undergoing slow mitoses. Results supp
ort the hypothesis that oxidative damage to long-lived post-mitotic ce
lls may be a key factor in the aging process.