Gm. Sanders et al., PRODUCTION OF MONOCLONAL-ANTIBODIES AGAINST XANTHOMONAS-CAMPESTRIS PVMANGIFERAEINDICAE AND THEIR USE TO INVESTIGATE DIFFERENCES IN VIRULENCE, Journal of Applied Bacteriology, 77(5), 1994, pp. 509-518
Four Xanthomonas campestris pv. mangiferaeindicae isolates from mango
black spot lesions were grouped according to differences in virulence
and used to raise monoclonal antibodies (mAbs). Two immunization appro
aches were followed. In the first, four groups of mice were immunized,
each with a different isolate and the spleens from each group homogen
ized together for cell fusion. The second approach entailed immunizati
on of a single group of mice with bacteria pooled from all four isolat
es. The resultant mAbs were characterized with regard to the antigen b
inding specificity and antibody class. A relationship between mAb bind
ing specificity and virulence of the bacteria was shown by Western blo
t analysis.