RAPID DETECTION OF PROLINE IMINOPEPTIDASE AS AN INDICATOR OF EIKENELLA-CORRODENS PERIODONTAL INFECTION

Authors
ALLAKER RP YOUNG KA HARDIE JM
Citation
Rp. Allaker et al., RAPID DETECTION OF PROLINE IMINOPEPTIDASE AS AN INDICATOR OF EIKENELLA-CORRODENS PERIODONTAL INFECTION, Letters in applied microbiology, 19(5), 1994, pp. 325-327
Citations number
9
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
19
Issue
5
Year of publication
1994
Pages
325 - 327
Database
ISI
SICI code
0266-8254(1994)19:5<325:RDOPIA>2.0.ZU;2-U
Abstract
A chromogenic test with L-proline p-nitroanilide as a substrate was de veloped to detect proline iminopeptidase activity from Eikenella corro dens. Optimal assay conditions for enzyme detection were 50 degrees C and pH 7.5-8.0. Forty paper point samples from adult periodontitis pat ients were screened for Eik. corrodens and proline iminopeptidase acti vity, to determine whether this enzyme is a marker for the pathogen. T wenty samples were positive for Eik. corrodens, and the levels of enzy me activity and populations of Eik. corrodens were found to correlate (P < 0.05).