IMPROVED ELUTION OF ISOAMYLASE ADSORBED ON RAW STARCH AND THE PRESERVATION OF PURIFIED ENZYME

The isoamylase from Pseudomonas amyloderamosa can be recovered by adso rption-elution on raw starch. In this process, the elution of adsorbed isoamylase was significantly affected by elution design. When the raw starch-enzyme suspension was packed into a funnel-type glass filter i nstead of being stirred in a flask to elute the adsorbed isoamylase, t he recovery was increased from 53.6% to about 81% and the concentratio n of isoamylase was also increased 42-fold in the eluted solution. The addition of 1% potassium sorbate into the eluted enzyme solution gave a retention of more than 80% of the recovered isoamylase activity in a 5-month storage at 4 degrees C.