Multiple mutagenesis of Fusarium oxysporum DSM 841 resulted in enhance
d yields of cellulases. The hypercellulolytic mutant (NTG-19) secretes
high levels of extracellular cellulases on different cellulosic subst
rates. Addition of surfactant, Tween-80, further increased enzyme secr
etion by about 30%. The results on hydrolysis of wheat straw by parent
strain, DSM 841 and mutant NTG-19 cellulases also revealed a signific
ant improvement in the hydrolytic potential of the cellulolytic enzyme
s from the mutant NTG-19.