A SIMPLE METHOD FOR IMMUNOFLUORESCENCE STAINING OF TRACHEAL ORGAN-CULTURES FOR THE RAPID IDENTIFICATION OF INFECTIOUS-BRONCHITIS VIRUS

Authors
BHATTACHARJEE PS NAYLOR CJ JONES RC
Citation
Ps. Bhattacharjee et al., A SIMPLE METHOD FOR IMMUNOFLUORESCENCE STAINING OF TRACHEAL ORGAN-CULTURES FOR THE RAPID IDENTIFICATION OF INFECTIOUS-BRONCHITIS VIRUS, Avian pathology, 23(3), 1994, pp. 471-480
Citations number
7
Categorie Soggetti
Veterinary Sciences
Journal title
Avian pathology → ACNP
ISSN journal
03079457
Volume
23
Issue
3
Year of publication
1994
Pages
471 - 480
Database
ISI
SICI code
0307-9457(1994)23:3<471:ASMFIS>2.0.ZU;2-2
Abstract
A method is described for the simple application of immunofluorescence (IF) staining for the identification of infectious bronchitis virus ( IBV) in tracheal organ cultures (TOC). It involves the application of antiserum and fluorescent antiglobulin to unfixed TOC. In TOC inoculat ed with high titres of virus, specific fluorescence was observed in le ss than 24 h. IF staining almost always occurred before ciliostasis. F ollowing experimental infection of chicks with IBV, virus could someti mes be detected in material horn tracheal or cloacal swabs within 24 h of inoculation of TOC.