INSTANT PRINS - A RAPID METHOD FOR CHROMOSOME IDENTIFICATION BY DETECTING REPEATED SEQUENCES IN-SITU

Authors
GOSDEN J LAWSON D
Citation
J. Gosden et D. Lawson, INSTANT PRINS - A RAPID METHOD FOR CHROMOSOME IDENTIFICATION BY DETECTING REPEATED SEQUENCES IN-SITU, Cytogenetics and cell genetics, 68(1-2), 1995, pp. 57-60
Citations number
10
Categorie Soggetti
Cytology & Histology","Genetics & Heredity
Journal title
Cytogenetics and cell genetics → ACNP
ISSN journal
03010171
Volume
68
Issue
1-2
Year of publication
1995
Pages
57 - 60
Database
ISI
SICI code
0301-0171(1995)68:1-2<57:IP-ARM>2.0.ZU;2-N
Abstract
We describe a method for labeling specific chromosomes in situ by olig onucleotide-primed synthesis and incorporation of fluorochrome-labeled dUTP, together with an accelerated protocol for the reaction. The com bination of the cases taking less than 5 min for the complete process. The significance of this development for clinical diagnosis is discus sed.