ASSIGNMENT OF 5 LOCI FROM HUMAN-CHROMOSOME-8Q ONTO SHEEP CHROMOSOME-9

Using a chromosomally characterized minipanel of sheep x hamster cell hybrids, five new loci, including carbonic anhydrase II (CA2), calbind in 1 (28 kDa) (CALB1), corticotropin releasing hormone (CRH), cytochro me P450 11B subfamily XIB (steroid-11-beta-hydroxylase), polypeptide 1 (CYP11B1), and interleukin 7 (IL7), have been assigned to sheep chrom osome 9. A homolog of CA2 was detected on sheep chromosome 1. CRH was regionally localized to sheep 9q23-->q28 by in situ hybridization. Thi s study assigns chromosome 9 as the sheep equivalent of cattle chromos ome 14 and indicates that CALB1, CYP11B1, and IL7, which have not been mapped on the cattle genome, are likely to be present on cattle chrom osome 14. It also shows by comparative genome analysis that a large se gment of human chromosome 8q is highly conserved in sheep chromosome 9 and cattle chromosome 14. Based on these data, we propose that sheep chromosome 9 be recognised as the equivalent of cattle chromosome 14.