A HIGH-RESOLUTION CYTOGENETIC MAP OF HUMAN-CHROMOSOME-2 - LOCALIZATION OF 434 COSMID MARKERS BY DIRECT R-BANDING FLUORESCENCE IN-SITU HYBRIDIZATION

We have constructed a high-resolution cytogenetic map of human chromos ome 2 with 434 newly isolated cosmid markers by means of direct R-band ing fluorescence in situ hybridization. Two markers were mapped to the centromeric region, 173 to the short arm, and 259 to the long arm. Th e clones were evenly distributed along the entire chromosome, although a tendency toward clustering in R-positive bands was observed. The ma pped cosmids provide useful landmarks for construction of a contig map and for positional cloning of cancer-associated genes, as well as gen es responsible for hereditary diseases.