Rg. Mills et al., THE SOLUTION STRUCTURE OF SARAFOTOXIN-C - IMPLICATIONS FOR LIGAND RECOGNITION BY ENDOTHELIN, The Journal of biological chemistry, 269(38), 1994, pp. 23413-23419
The solution structure of sarafotoxin-c has been determined using NMR
spectroscopy. A total of 112 interproton distance constraints derived
from two-dimensional MMR spectra were used to calculate a family of st
ructures using a combination of distance geometry and dynamical simula
ted annealing calculations. The structures reveal a well defined cu he
lix extending from Glu(9) to Cys(15) and an N-terminal region (Cys(1)-
Asp(8)) that is tightly constrained by disulfide bands to Cys residues
in the central helix. In contrast, the C-terminal region (His(16)-Trp
(21)) does not adopt a defined conformation in the final family of str
uctures. This is consistent with the paucity of NMR-derived structural
constraints obtained for this region and leads to the suggestion that
the C-terminal region oscillates rapidly between a number of substant
ially different conformers. It is proposed that differences between th
e central helix of the endothelin and sarafotoxin isopeptides might be
important in binding of these ligands by the G protein-coupled endoth
elin receptors.