ANALYSIS IN GREENHOUSE OF POTATO PLANTS T RANSFORMED BY AGROBACTERIUM-RHIZOGENES

A morphological study of potato plants transformed by Agrobacterium rh izogenes (mannopine and cucumopine strains) was conducted in the green house. A Dot Blot analysis confirmed the integration of the Ri plasmid T-DNA in these plants. The transformed plants obtained by regeneratio n from transformed root lines or directly formed on internode sections showed a great variation in their phenotypes. Several variation level s have been observed: variation was less extended between plants issue d from a same root line than between plants regenerated from independe nt root lines. Moreover, plants directly produced on internode section s were morphologically less disturbed. This new transformation process seems, therefore, to limit somaclonal variation.