PSORALEN TREATMENT OF ADENOVIRUS PARTICLES ELIMINATES VIRUS-REPLICATION AND TRANSCRIPTION WHILE MAINTAINING THE ENDOSOMOLYTIC ACTIVITY OF THE VIRUS CAPSID
M. Cotten et al., PSORALEN TREATMENT OF ADENOVIRUS PARTICLES ELIMINATES VIRUS-REPLICATION AND TRANSCRIPTION WHILE MAINTAINING THE ENDOSOMOLYTIC ACTIVITY OF THE VIRUS CAPSID, Virology, 205(1), 1994, pp. 254-261
Adenovirus entry into its host cell transiently permeabilizes the cell
allowing the coentry of reagents such as DNA. We compare here adenovi
rus inactivation with beta-propiolactone and several psoralen derivati
ves, seeking reagents that disrupt the viral genome without impairing
the viral entry functions. No virus replication can be detected after
8-methoxypsoralen (8-MOP) modification. Viral transcription is not det
ectable by Northern analysis, and reverse transcriptase/PCR analysis d
emonstrates at least a 1000-fold decrease in viral transcription after
8-MOP treatment. Using [H-3]8-MOP, the psoralen is found to enter the
virus capsid and react throughout the viral genome, with approximatel
y one psoralen modification per 100 bp of viral DNA. This inactivated
adenovirus allows us to deliver DNA to target cells without interferen
ce from adenovirus gene expression or replication. Furthermore, we can
now study the host cell response to adenovirus entry without the comp
lications of adenovirus gene expression. (C) 1994 Academic Press, Inc.