THE IMMUNOHISTOCHEMICAL DISTRIBUTION OF PROTEIN-KINASE-C ISOZYMES IS ALTERED IN THE CANINE BRAIN AND BASILAR ARTERY AFTER SUBARACHNOID HEMORRHAGE

The effects of subarachnoid hemorrhage on immunoreactivities of protei n kinase C isozymes (alpha, beta, gamma) were studied in canine brain and basilar artery. Mild and severe constriction of the basilar artery was shown 2 days and 7 days after hemorrhage, respectively. In contro l brain tissues, the isozymes showed distinct distributions and follow ing hemorrhage, reactive astrocytes with protein kinase C alpha staini ng emerged in the surface of the pens and hippocampus. The control bas ilar artery showed protein kinase C alpha staining and its staining wa s decreased on day 7, consistent with the result of immunoblot. Our re sults demonstrated that subarachnoid hemorrhage induces gliosis with h eavy protein kinase C alpha staining and down-regulation of protein ki nase C in the artery.