Several physiological studies have shown that the subcommissural organ
(SCO) is influenced by catecholamines. This study provides immunohist
ochemical evidence for a noradrenergic input to the SCO of rats. A lig
ht plexus of tyrosine hydroxylase (TH)- and dopamine-beta-hydroxylase
(D beta H)-positive axons present in the SCO of both Long-Evans and Sp
rague-Dawley rats. The innervation density was greatest in the hypendm
yal wing of the rostral aspect of the SCO and it declined both caudall
y in the hypendymal wing and medially in the hypendymal layer. Some TH
- and D beta H-immunoreactive fibers entered the lateral margin of the
ependymal layer along the basal surface of ependymal cells; others co
ursed medially in the transverse plane to ramify along the base of the
ependymal cells. These fibers are presumed to be noradrenergic becaus
e phenylethanolamine N-methyltransferase immunoreactivity was absent i
n adjacent sections through the SCO. Considering the potential role of
the SCO region in sodium homeostasis, these data suggest that central
noradrenergic input to the SCO may parallel peripheral catecholaminer
gic mechanisms that regulate sodium balance.