MITOGENIC AND CHEMOTACTIC RESPONSES OF HUMAN PERIODONTAL-LIGAMENT CELLS TO THE DIFFERENT ISOFORMS OF PLATELET-DERIVED GROWTH-FACTOR

A major focus of studies that center on regeneration of the periodonti um is to determine the efficacy of the use of polypeptide growth facto rs. Platelet-derived growth factor has been reported to be a possible agent for clinical use. PDGF has various isoforms. Therefore, we decid ed to study the mitogenic and chemotactic responses of human periodont al ligament (PDL) cells to recombinant human PDGF-AB, AA, and BB. Addi tion of each isoform of PDGF to in vitro mitogenesis assays induced PD L cell proliferation in a dose-dependent manner. The maximum mitogenic effect was evident at the concentration of 100 ng/mL. In these assays , PDGF-BB was found to be the most potent mitogen. PDGF-AB elicited an intermediate response, and PDGF-AA was the least effective. The resul ts of chemotaxis assays closely parallel those of the mitogenesis assa ys. PDGF-BB exhibited the most potent chemotactic effect. The maximal effect was observed at 10 ng/mL. The findings of these experiments ind icate that PDGF-BB is more effective than the other isoforms in promot ing mitogenesis and chemotaxis of PDL cells in vitro, and may therefor e be a suitable ethical pharmaceutical for use in periodontal regenera tion procedures.