CHIRAL SEPARATION OF DINICONAZOLE, UNICONAZOLE AND STRUCTURALLY RELATED-COMPOUNDS BY CYCLODEXTRIN-MODIFIED MICELLAR ELECTROKINETIC CHROMATOGRAPHY

Enantiomeric separation of the structural analogs, diniconazole and un iconazole, can be achieved by cyclodextrin (CD)-modified micellar elec trokinetic chromatography (MEKC). The elution behavior of other struct urally related compounds was therefore investigated and compared with that in highperformance liquid chromatography (HPLC) using a CD-bonded stationary phase. From the results, it was concluded that the benzene ring of the solute plays an important role in formation of CD complex es and chiral recognition in MEKC as well as in HPLC, although the ena ntioselectivity with the two methods was different. Nuclear magnetic r esonance (NMR) data revealed different CD effects on the solute betwee n the MEKC and HPLC models.