ENANTIOMERIC SEPARATION OF DRUGS BY AFFINITY ELECTROKINETIC CHROMATOGRAPHY USING DEXTRAN SULFATE

Dextran sulfate (sodium salt), which is a mixture of a linear alpha-(1 ,6)-linked D-glucose polymer having a sulfate group in the molecule, h as been employed as a chiral selector in affinity electrokinetic chrom atography (EKC) for the separation of enantiomers of drugs. Enantiomer s of trimetoquinol hydrochloride and its positional isomer have been s uccessfully separated by the method using a 3-5% dextran sulfate buffe r solution of pH 5.5 within 20 min. The effects of buffer pH, concentr ation of dextran sulfate, and additives (such as an organic solvent, u rea and a surfactant) on the enantioselectivity were investigated. The choice of pH and the concentration of dextran sulfate has been import ant for the improvement in selectivity.