THE CHARACTERIZATION AND CRYSTALLIZATION OF A VIRALLY ENCODED USTILAGO-MAYDIS KP4 TOXIN

Authors
GU F SULLIVAN TS CHE ZW GANESA C FLURKEY WH BOZARTH RF SMITH TJ
Citation
F. Gu et al., THE CHARACTERIZATION AND CRYSTALLIZATION OF A VIRALLY ENCODED USTILAGO-MAYDIS KP4 TOXIN, Journal of Molecular Biology, 243(4), 1994, pp. 792-795
Citations number
39
Categorie Soggetti
Biology
Journal title
Journal of Molecular Biology → ACNP
ISSN journal
00222836
Volume
243
Issue
4
Year of publication
1994
Pages
792 - 795
Database
ISI
SICI code
0022-2836(1994)243:4<792:TCACOA>2.0.ZU;2-6
Abstract
KP4 is a virally encoded and highly specific toxin that kills fungi cl osely related to the fungus Ustilago maydis. The toxin was purified an d, crystals were formed using ammonium sulfate as precipitant. The cry stals belong to the space group P6(1(5))22 and diffracted to similar t o 2.2 Angstrom resolution. Circular dicroism spectroscopy suggests tha t the protein is predominantly comprised of beta-strands.