CHARACTERISTICS OF CARROT PLANT REGENERATED FROM 2 CELL-LINE SELECTEDAS EITHER IONIC-AL TOLERANT CELL OR AL-PHOSPHATE UTILIZING CELLS

Plantlets of carrot (Daucus carota L.) were regenerated from two types of cell lines. One type was selected as ionic-Al tolerant (IAT) cells , while the second type featured Al-phosphate utilizing cells (IPG). T heir tolerance characteristics were investigated. The plantlets from I AT were directly regenerated, whereas those from IPG were regenerated after somatic hybridization with wild-type cells previously inactivate d with iodacetamide, because the IPG cells had completely lost the abi lity to regenerate naturally. The sexual progeny of IAT showed Al-tole rant properties, established by testing their root elongation in the p resence of 500 mu M Al ions. Most of the calli obtained from the somat ic hybrids grew more rapidly than the wild-type cells when Al-phosphat e was used as a sole source of phosphorus. Thus, we obtained two types of carrot plantlets, regenerated from IAT and IPG. Both possessed the tolerant characteristics as observed with the stress-selected cells.