STRUCTURAL POLYPEPTIDES OF DIFFERENT CLINICAL STRAINS OF AVIAN ADENOVIRUS TYPE-1

The polypeptides of two liver strains, two ovarian strains and one sta ndard strain of avian adenovirus-1 (AAV-1) were analysed by sodium dod ecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western b lot using polyclonal antibodies to all the strains and isoelectric foc using (IEF). All the strains had 11 polypeptides and molecular weight varied between 9 and 100 kDa. The polypeptide pattern was similar in a ll the strains as revealed by SDS-PAGE and Western blot. However, some differences among the strains on the basis of isoelectric point of po lypeptide were observed by IEF. (C) 1996 Elsevier Science Ltd.