Nk. Maiti et P. Sarkar, STRUCTURAL POLYPEPTIDES OF DIFFERENT CLINICAL STRAINS OF AVIAN ADENOVIRUS TYPE-1, Comparative immunology, microbiology and infectious diseases, 20(1), 1997, pp. 53-58
The polypeptides of two liver strains, two ovarian strains and one sta
ndard strain of avian adenovirus-1 (AAV-1) were analysed by sodium dod
ecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Western b
lot using polyclonal antibodies to all the strains and isoelectric foc
using (IEF). All the strains had 11 polypeptides and molecular weight
varied between 9 and 100 kDa. The polypeptide pattern was similar in a
ll the strains as revealed by SDS-PAGE and Western blot. However, some
differences among the strains on the basis of isoelectric point of po
lypeptide were observed by IEF. (C) 1996 Elsevier Science Ltd.