PURIFICATION AND CHARACTERIZATION OF INVERTASE FROM ASPERGILLUS-NIGER

Authors
RUBIO MC MALDONADO MC
Citation
Mc. Rubio et Mc. Maldonado, PURIFICATION AND CHARACTERIZATION OF INVERTASE FROM ASPERGILLUS-NIGER, Current microbiology, 31(2), 1995, pp. 80-83
Citations number
15
Categorie Soggetti
Microbiology
Journal title
Current microbiology → ACNP
ISSN journal
03438651
Volume
31
Issue
2
Year of publication
1995
Pages
80 - 83
Database
ISI
SICI code
0343-8651(1995)31:2<80:PACOIF>2.0.ZU;2-P
Abstract
Invertase produced by a strain of Aspergillus niger showed the followi ng main characteristics: maximum activity at 60 degrees C, pH 5.0; K-m with sucrose as substrate, 0.0625 mM; V-max 0.013 mol/min; and free e nergy 9132 cal/mol. The metal ions and p-chloromercuribenzoate (PCMB) acted as inhibitors respectively.