THE DERIVATION OF BINDING PARAMETERS FROM EFFECTOR AND TARGET CONJUGATE FREQUENCY DATA USING LINEAR AND NONLINEAR DATA-FITTING TRANSFORMATIONS - APPLICATION OF SUCH TRANSFORMATIONS TO THE NK-MOLT4 AND NK-K562 EFFECTOR-TARGET SYSTEMS

Effector-target conjugation is described quantitatively by binding iso therms which are characterized by three parameters, the maximum effect or and target conjugate frequencies, alpha(max) and beta(max), and the dissociation constant of the conjugates formed, K-D. In this paper th e application of non-linear data-fitting techniques, as well as linear transformations of the binding isotherms that permit us to use standa rd regression analysis, has been tested to calculate estimates of thes e parameters in the NK-MOLT4 and NK-K562 effector-target systems. Both unweighted and weighted data were used to calculate alpha(max), beta( max) and K-D for six different donors which were used as a source of N K cells. The results obtained have shown that these regression methods are useful for revealing potential disparities between binding effici encies in effector-target systems.